Group I Introns Found in Chlorella Viruses: Biological Implications
Identifieur interne : 003B65 ( Main/Exploration ); précédent : 003B64; suivant : 003B66Group I Introns Found in Chlorella Viruses: Biological Implications
Auteurs : Kensho Nishida [Japon] ; Satoshi Suzuki [Japon] ; Yoshiki Kimura [Japon] ; Nobuhiko Nomura [Japon] ; Makoto Fujie [Japon] ; Takashi Yamada [Japon]Source :
- Virology [ 0042-6822 ] ; 1998.
English descriptors
- Teeft :
- Alga, Capsid, Cech, Chlorella, Chlorella virus genome, Chlorella virus group, Chlorella viruses, Cven5, Cvk2, Cvt2, Cvu1, Endonuclease, Endonuclease gene, Etten, Exon, Exon genes, Fungi, Gene, Genome, Hybridization, Hybridizing, Hydra viridis, Initiation codon, Intron, Intron probe, Intron sequence, Intron sequences, Kutish, Major capsid protein, Meints, Middle portion, Nishida, Nucleotide, Nucleotide positions, Nucleotide sequence, Nucleotide sequences, Phylogenetic, Phylogenetic analysis, Plaque, Plaque hybridization, Pneumocystis carinii, Primary sequence, Rrna, Rrna genes, Secondary structure, Southern blot analysis, Tfiis, Tfiis gene, Viral, Virology, Virus, Weak bands, Yamada.
Abstract
Abstract: More than 80 group I introns were detected and characterized inChlorellaviruses isolated from various locations in Japan; the overall average frequency of viruses containing the group I intron was 8.0%. Although most of these introns were inserted in the gene for either transcriptional elongation factor TFIIS (∼60%) or URF14.2 (unidentified open reading frame coding for a 14.2-kDa polypeptide) (∼40%), in a few cases, the gene for the major capsid protein Vp52 contained an intron. These introns were biologically active (self-splicing) bothin vivoandin vitro.Viruses that contained introns almost usually contained only one, but more than two introns coexisted in several virus isolates. Nucleotide sequence analysis showed that the intron sequences have diverged under strong constraint of the exon genes: introns in the same gene showed more than 99% sequence identity, whereas introns in different genes were only 72–78% identical. Phylogenetic analysis suggested relatedness of these introns to those found in the rRNA genes of a variety of organisms including green algae, red algae, yeasts, fungi, and protozoa.
Url:
DOI: 10.1006/viro.1998.9030
Affiliations:
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sort="Kimura, Yoshiki" uniqKey="Kimura Y" first="Yoshiki" last="Kimura">Yoshiki Kimura</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739</wicri:regionArea><wicri:noRegion>739</wicri:noRegion></affiliation></author><author><name sortKey="Nomura, Nobuhiko" sort="Nomura, Nobuhiko" uniqKey="Nomura N" first="Nobuhiko" last="Nomura">Nobuhiko Nomura</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739</wicri:regionArea><wicri:noRegion>739</wicri:noRegion></affiliation></author><author><name sortKey="Fujie, Makoto" sort="Fujie, Makoto" uniqKey="Fujie M" first="Makoto" last="Fujie">Makoto Fujie</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739</wicri:regionArea><wicri:noRegion>739</wicri:noRegion></affiliation></author><author><name sortKey="Yamada, Takashi" sort="Yamada, Takashi" uniqKey="Yamada T" first="Takashi" last="Yamada">Takashi Yamada</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739</wicri:regionArea><wicri:noRegion>739</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">242</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="319">319</biblScope><biblScope unit="page" to="326">326</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alga</term><term>Capsid</term><term>Cech</term><term>Chlorella</term><term>Chlorella virus genome</term><term>Chlorella virus group</term><term>Chlorella viruses</term><term>Cven5</term><term>Cvk2</term><term>Cvt2</term><term>Cvu1</term><term>Endonuclease</term><term>Endonuclease gene</term><term>Etten</term><term>Exon</term><term>Exon genes</term><term>Fungi</term><term>Gene</term><term>Genome</term><term>Hybridization</term><term>Hybridizing</term><term>Hydra viridis</term><term>Initiation codon</term><term>Intron</term><term>Intron probe</term><term>Intron sequence</term><term>Intron sequences</term><term>Kutish</term><term>Major capsid protein</term><term>Meints</term><term>Middle portion</term><term>Nishida</term><term>Nucleotide</term><term>Nucleotide positions</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Plaque</term><term>Plaque hybridization</term><term>Pneumocystis carinii</term><term>Primary sequence</term><term>Rrna</term><term>Rrna genes</term><term>Secondary structure</term><term>Southern blot analysis</term><term>Tfiis</term><term>Tfiis gene</term><term>Viral</term><term>Virology</term><term>Virus</term><term>Weak bands</term><term>Yamada</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: More than 80 group I introns were detected and characterized inChlorellaviruses isolated from various locations in Japan; the overall average frequency of viruses containing the group I intron was 8.0%. Although most of these introns were inserted in the gene for either transcriptional elongation factor TFIIS (∼60%) or URF14.2 (unidentified open reading frame coding for a 14.2-kDa polypeptide) (∼40%), in a few cases, the gene for the major capsid protein Vp52 contained an intron. These introns were biologically active (self-splicing) bothin vivoandin vitro.Viruses that contained introns almost usually contained only one, but more than two introns coexisted in several virus isolates. Nucleotide sequence analysis showed that the intron sequences have diverged under strong constraint of the exon genes: introns in the same gene showed more than 99% sequence identity, whereas introns in different genes were only 72–78% identical. Phylogenetic analysis suggested relatedness of these introns to those found in the rRNA genes of a variety of organisms including green algae, red algae, yeasts, fungi, and protozoa.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Nishida, Kensho" sort="Nishida, Kensho" uniqKey="Nishida K" first="Kensho" last="Nishida">Kensho Nishida</name></noRegion><name sortKey="Fujie, Makoto" sort="Fujie, Makoto" uniqKey="Fujie M" first="Makoto" last="Fujie">Makoto Fujie</name><name sortKey="Kimura, Yoshiki" sort="Kimura, Yoshiki" uniqKey="Kimura Y" first="Yoshiki" last="Kimura">Yoshiki Kimura</name><name sortKey="Nomura, Nobuhiko" sort="Nomura, Nobuhiko" uniqKey="Nomura N" first="Nobuhiko" last="Nomura">Nobuhiko Nomura</name><name sortKey="Suzuki, Satoshi" sort="Suzuki, Satoshi" uniqKey="Suzuki S" first="Satoshi" last="Suzuki">Satoshi Suzuki</name><name sortKey="Yamada, Takashi" sort="Yamada, Takashi" uniqKey="Yamada T" first="Takashi" last="Yamada">Takashi Yamada</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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